Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin
نویسندگان
چکیده
منابع مشابه
Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin.
DNA complementary to calf stomach mRNA has been synthesised and inserted into the Pst1 site of pAT153 by G-C tailing. Clones containing sequences coding for prochymosin were recognised by colony hybridisation with cDNA extended from a chemically synthesised oligodeoxynucleotide primer, the sequence of which was predicted from the published amino acid sequence of calf prochymosin. Two clones wer...
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Prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. In the present investigation we report sequence of cDNA encoding goat ( Capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. As bovine prochymosin, the caprine prochymosin cDNA encodes 365 amino ...
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متن کاملnucleotide sequence of cdna encoding for preprochymosin in native goat (capra hircus) from iran
prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. in the present investigation we report sequence of cdna encoding goat ( capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. as bovine prochymosin, the caprine prochymosin cdna encodes 365 amino ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1982
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/10.7.2177